SOLVED: How can DNA fragments from restriction digest analysisand plasmid DNA conformations be validated via gelelectrophoresis?

SOLVED: You have performed a restriction digest on a linear 10.5 Kbp piece of DNA. The sizes of the DNA fragments resulting from the different restriction digests are shown below. Using this information, construct a linear map of this DNA illustrating the location(s) of the different restriction enzyme sites and the distances between each site. (20 pts)Enzyme Size of Fragments Produced (Kbp)BamHI 6.5, 2.5, 1.5EcoRI 5.5, 3.0, 2.0SalI 10.0, 0.5HindIII 8.5, 2.0BamHI/EcoRI 4.0, 2.5, 2.0, 1.5, 0.5Bam

SOLVED: You want to set up a restriction enzyme digest of your isolated plasmid, pMICO. You determine that the concentration of your plasmid DNA is 50 nanograms/microliter. How many microliters of your purified plasmid DNA would you add to a 20 microliter digest in order to add 250 nanograms DNA to the digest?

Difference between EcoRI and HindIII Restriction Enzymes

Texts: #1-4 are restriction enzymes and the DNA sequence that they recognize. Which one of the enzymes below could cut the following DNA sequence? Assume the sequence is in a larger piece of DNA. 5' TCGATGAAGCTTG 3'1.) AatII GACGTC2.) BamHI GGATC3.) EcoRI GAATTC4.) HindIII AAGCTT5.) XhoI CTCGAG

complete the following problems restriction enzymes res which cut d na at specific sequences are classic tools in molecular biology because of their specificity in cutting dna res can be used to map d