Leader cells promote immunosuppression to drive ovarian cancer ...
ID8 cells were stimulated with 1x104UI/ml IFNβ for 3 h. Supernatants were collected and centrifuged to remove debris. Chemokines in IFNβ-stimulated ID8 cell supernatants, mouse serum (diluted 1:4) and mouse peritoneal washes were quantified using the Bio-Plex Pro Mouse Chemokine Panel 33-Plex (Bio-Rad #12002231) assay, according to the ...
Cellosaurus cell line ID8 (CVCL_IU14)
Cell type: Epithelial cell of ovary; CL= CL_2000064. Breed/subspecies: C57BL/6. Development of a syngeneic mouse model for events related to ovarian cancer. Comparison of ID8 MOSE and VEGF-modified ID8 cell lines in an immunocompetent animal model for human ovarian cancer.
Genetically defined syngeneic mouse models of ovarian cancer as …
In contrast, syngeneic models, such as the commonly used ID8 murine model , together with genetically modified versions of these cells (12,13), have been extensively used to investigate the roles of the immune system in HGSC progression and to study therapeutic responses.
ID8 cell line is a highly published and well characterized cell line and is frequently used as a syngeneic mouse model for ovarian cancer. Storage and Handling. ID8 Mouse Ovarian Epithelial Cells should be stored in liquid nitrogen.
ID8 Mouse Ovarian Surface Epithelial Cell Line - MilliporeSigma
ID8 cell line is a highly published and well characterized cell line and is frequently used as a syngeneic mouse model for ovarian cancer. Ovarian cancer is the fourth leading cause of cancer-related deaths in women.
Personalized cancer vaccine strategy elicits polyfunctional T cells …
Mar 15, 2021 · In the ID8 ovarian model, animals receiving the same regimen showed prolonged survival, increased tumor-infiltrating perforin-producing T cells, increased neoantigen-specific CD8 + T cells,...
ID8 Mouse Ovarian Surface Epithelial Cell Line | SCC145 - EMD …
Of the 10 clonal lines, ID8 exhibited the highest tumor load. ID8 cell line is a highly published and well characterized cell line and is frequently used as a syngeneic mouse model for ovarian cancer. Ovarian cancer is the fourth leading cause of cancer-related deaths in women.
CRISPR/Cas9-derived models of ovarian high grade serous
Dec 4, 2017 · First described in 2000 13, the ID8 model is a widely-used syngeneic model of ovarian cancer. However, using whole-exome sequencing, we recently showed that ID8 lacked any mutations fundamental...
Ovarian cancer mouse models: a summary of current models and …
Sep 28, 2009 · Greenaway et al. injected a spontaneously tumorigenic MOSE cell line, ID8, into the ovarian bursal cavity of C57Bl6 mice. The ID8 cells formed direct contact with the ovarian stroma, resulting in primary tumor formation, secondary peritoneal carcinomatosis, and extensive ascites fluid production between 80 to 90 days post-exposure.
Preclinical models of epithelial ovarian cancer: practical ...
Jun 16, 2022 · Currently used models for EOC include the commonly used ID8 murine model together with genetically modified versions of these cells [97,98,99,100,101]. The syngeneic mouse model permits EOC initiation directly from mouse OSE (MOSE).