The cells treated with the siRNAs against c-Abl show increased TFEB-GFP nuclear localization compared with cells transfected with a scramble siRNA (Figure 1F), demonstrating that c-Abl depletion promotes TFEB nuclear translocation.

In this review article, we focus on how phosphorylation‐mediated signaling pathways regulate the subcellular localization and function of transcription factor EB (TFEB) and the other members of the MiT‐TFE family.

Recent studies show that TFEB and TFE3 function in innate immune cells to regulate antibacterial and antiviral responses downstream of phagocytosis, IFN-γ, LPS, and adenosine receptors.

Dec 16, 2024 · STING activation enhanced lysosome biogenesis through inducing the nuclear translocation of transcription factor EB (TFEB) as well as its paralogs transcription factor E3 (TFE3) and microphthalmia-associated transcription factor (MITF).

TFEB siRNA (m), shRNA and Lentiviral Particle Gene Silencers designed to knockdown mouse TFEB gene expression. Cited in 16 publications.

Jun 12, 2012 · To further test the role of mTOR in regulating TFEB localization, we performed small interfering RNA (siRNA)–mediated knockdowns of mTOR and RagC, a guanosine triphosphatase (GTPase) that recruits the mTORC1 complex to lysosomes (26, 28).

Aug 28, 2018 · Most recently, the transcription factor EB (TFEB) is identified as a master regulator for autophagic flux via initiating the expression of multiple autophagy-related genes and lysosomal...

Mar 19, 2015 · Small interfering RNA (siRNA) was used to silence TFEB expression in H4/α-syn-GFP cells and the accumulation of α-syn-GFP aggregates was analyzed by confocal microscopy. Treatment with TFEB siRNA resulted in 60% reduction in TFEB expression compared to cells

Jun 22, 2021 · Confirming the original screen findings, we used MCF-7 cells stably expressing the autophagy marker green fluorescent protein (GFP)-LC3B and observed that small interfering RNA (siRNA)-mediated...

Mar 22, 2021 · Transcription factor EB (TFEB) is a well-known master regulator of autophagy and lysosomal biogenesis processes. TFEB’s nuclear localization and transcriptional activity are also regulated by...