Zeolitic imidazolate frameworks (ZIFs) are a class of metal-organic frameworks (MOFs) that are topologically isomorphic with zeolites. [1] . ZIFs are composed of tetrahedrally-coordinated transition metal ions (e.g. Fe, Co, Zn) connected by imidazolate linkers.

Nov 1, 2023 · Our findings reveal that ZIF-1 transitions from degrading CCCH Zn finger proteins in somatic cells to clearing them from undifferentiated germ cells, and that ZIF-1 activity can be harnessed as a new genetic tool to study the early germline.

Abstract: Twelve zeolitic imidazolate frameworks (ZIFs; termed ZIF-1 to -12) have been synthesized as crystals by copolymerization of either Zn (II) (ZIF-1 to -4, -6 to -8, and -10 to -11) or Co (II) (ZIF-9 and -12) with imidazolate-type links.

We harness germline ZIF-1 activity to degrade a ZF1-tagged fusion protein from PGCs and show that its depletion produces phenotypes equivalent to those of a null mutation.

The comprehensive elastic properties of Zeolitic Imidazolate Frameworks (ZIF-1 to ZIF-4) have been computed using density functional theory (DFT).

With this approach, target proteins are tagged with a 36 amino acid degron sequence (ZF1); the SOCS-box adaptor protein ZIF-1, which targets ZF1-containing proteins for proteasomal degradation, is expressed in the target tissue (Armenti et al., 2014).

Twelve zeolitic imidazolate frameworks (ZIFs; termed ZIF-1 to -12) have been synthesized as crystals by copolymerization of either Zn(II) (ZIF-1 to -4, -6 to -8, and -10 to -11) or Co(II) (ZIF-9 and -12) with imidazolate-type links.

ZF-degron, GFP, and 3xFLAG tags inserted into endogenous air-1 locus. No overt phenotypes in a zif-1 (gk117) background. GFP expression is observed in mitotic cells at the spindle poles and along microtubules. Presence of ZF-degron targets tagged proteins for ZIF-mediated degradation.

Aug 2, 2019 · To test endogenous degrons, we primarily used the zinc finger 1 (ZF1) degron from the PIE-1 protein to degrade fluorescent reporters in developing C. elegans embryos. The ZF1 degron is a 36...

Oct 10, 2010 · zif-1 encodes the substrate-binding subunit of the E3 ligase for PIE-1 degradation. Inhibition of zif-1 translation in oocytes ensures high PIE-1 levels in oocytes and germline blastomeres. The two OMA protein functions are strictly regulated in both space and time by MBK-2, a kinase activated following fertilization.