Yellow fluorescent protein (YFP) is a genetic mutant of green fluorescent protein (GFP) originally derived from the jellyfish Aequorea victoria. [1] Its excitation peak is 513 nm and its emission peak is 527 nm. [2]

Aug 28, 1998 · We have determined the crystal structures of YFP T203Y/S65G/V68L/S72A and YFP H148G to 2.5 and 2.6 A resolution, respectively. Both structures show clear electron density for nearly coplanar pi-pi stacking between Tyr203 and the chromophore.

Sep 1, 2005 · CFP–YFP FRET is a stringent and reliable indicator of in vivo protein–protein interactions that allows the steady-state association of functional fusion proteins to be monitored at their natural site of subcellular localization in unfixed cells.

Mar 12, 2021 · I am currenlty studying the GFP varient, YFP and to my understanding, the mutation T203Y produces a face-to-face pi stacking interaction between the aromatic rings of both tyrosines (the chromophore and residue 203 mutant tyrosine).

Nov 1, 2024 · In this report we demonstrated that the 405-nm violet laser excitation could be used to discriminate GFP and YFP signals. Using the violet laser to distinguish GFP and YFP has several advantages. First, the standard setup of the flow cytometer could be used, obviating the need for exchanging filters and dichroic mirrors.

Yellow fluorescent protein (YFP) dyes are a class of fluorescent proteins that emit yellow fluorescence when excited by light in the blue to green range. These dyes are derived from the green fluorescent protein (GFP) family, which was originally isolated from the …

Aug 17, 2017 · The π-stacking contribution to the absorption red shift observed beween GFP and YFP is quantified. We compare the π -stacking energy landscapes in the S 0 and S 1 states, and examine the validity of a standard force field description.

Named after its proficiency in FRET (YFP for e nergy t ransfer), YPet is the brightest yellow fluorescent protein variant yet developed and demonstrates very good photostability. The resistance to acidic environments afforded by YPet is superior to Venus and other YFP derivatives, which will enhance the utility of this probe in biosensor ...

CFP eGFP YFP PI Figure 1: The MACSQuant VYB can detect a wide range of fluorescent proteins. Histograms of cells transfected with CFP, eGFP, YFP (top row), non-transfected cells (shown in purple). GFP and YFP are often difficult to distinguish using flow cytometry due to similarities in their emission spectra.

Yellow fluorescent protein (YFP) is a genetic mutant of green fluorescent protein (GFP) originally derived from the jellyfish Aequorea victoria. Its excitation peak is 514 nm and its emission peak is 527 nm. Like the parent GFP, YFP is a useful tool in cell and molecular biology due to its properties useful for fluorescence microscopy.