
Mammalian Atg8 proteins and the autophagy factor IRGM control …
Aug 3, 2020 · Here, we show that mammalian Atg8 proteins (mAtg8s) and the autophagy regulator IRGM control TFEB, a transcriptional activator of the lysosomal system. IRGM …
Rag GTPases control TFEB subcellular localization. (A–C ...
(A–C) Immunofluorescence of HEK-293T cells that express TFEB–3 × FLAG along with a control GTPase or the indicated Rag mutants.
TFEB overexpression in HEK-293 APPswe or C99 expressing
TFEB overexpression in HEK-293 APPswe or C99 expressing cells leads to the induction of numerous lysosomal-autophagic genes and to reduced C99 levels. (A) Bars show the …
TFEB regulates pluripotency transcriptional network in mouse …
However, in the present study, we propose a novel mechanism for TFEB governing pluripotency of mouse ESCs (mESCs) by regulating the pluripotency transcriptional network (PTN) in these …
Deacetylation of TFEB promotes fibrillar Aβ degradation by …
To investigate acetylation status of TFEB, Flag-tagged human TFEB was transiently transfected into human embryonic kidney (HEK) 293T cells and its acetylation level was detected by an …
Mammalian Atg8 proteins and autophagy factor IRGM control mTOR and TFEB ...
Here we show that mammalian Atg8 proteins (mAtg8s) and the autophagy regulator IRGM control TFEB, a transcriptional activator of the lysosomal system. IRGM directly interacted with TFEB …
Rag GTPases mediate amino acid–dependent recruitment of TFEB …
Feb 2, 2013 · Active Rag GTPases are required for recruitment of TFEB to lysosomes and its phosphorylation by mTORC1, inhibiting its function under nutrient-rich conditions. The …
TFEB SUMOylation in macrophages accelerates atherosclerosis by ...
Nov 11, 2023 · To verify the influence of TFEB SUMOylation on the interaction between TFEB and FACT complex observed in MS analysis, we used sodium arsenite (NaAsO 2) to stimulate …
A lysosome‐to‐nucleus signalling mechanism senses and regulates …
Feb 17, 2012 · TFEB subcellular localization was analysed in HeLa and HEK‐293T cells transiently transfected with a TFEB–3 × FLAG plasmid and treated overnight with several …
MCOLN1 is a ROS sensor in lysosomes that regulates autophagy
Jun 30, 2016 · Here, the authors show that ROS can induce autophagy by triggering TRPML1 to release Ca2+from the lysosomal lumen, in turn activating the autophagy and lysosomal …