Aug 3, 2020 · Here, we show that mammalian Atg8 proteins (mAtg8s) and the autophagy regulator IRGM control TFEB, a transcriptional activator of the lysosomal system. IRGM …

(A–C) Immunofluorescence of HEK-293T cells that express TFEB–3 × FLAG along with a control GTPase or the indicated Rag mutants.

TFEB overexpression in HEK-293 APPswe or C99 expressing cells leads to the induction of numerous lysosomal-autophagic genes and to reduced C99 levels. (A) Bars show the …

However, in the present study, we propose a novel mechanism for TFEB governing pluripotency of mouse ESCs (mESCs) by regulating the pluripotency transcriptional network (PTN) in these …

To investigate acetylation status of TFEB, Flag-tagged human TFEB was transiently transfected into human embryonic kidney (HEK) 293T cells and its acetylation level was detected by an …

Here we show that mammalian Atg8 proteins (mAtg8s) and the autophagy regulator IRGM control TFEB, a transcriptional activator of the lysosomal system. IRGM directly interacted with TFEB …

Feb 2, 2013 · Active Rag GTPases are required for recruitment of TFEB to lysosomes and its phosphorylation by mTORC1, inhibiting its function under nutrient-rich conditions. The …

Nov 11, 2023 · To verify the influence of TFEB SUMOylation on the interaction between TFEB and FACT complex observed in MS analysis, we used sodium arsenite (NaAsO 2) to stimulate …

Feb 17, 2012 · TFEB subcellular localization was analysed in HeLa and HEK‐293T cells transiently transfected with a TFEB–3 × FLAG plasmid and treated overnight with several …

Jun 30, 2016 · Here, the authors show that ROS can induce autophagy by triggering TRPML1 to release Ca2+from the lysosomal lumen, in turn activating the autophagy and lysosomal …