To investigate acetylation status of TFEB, Flag-tagged human TFEB was transiently transfected into human embryonic kidney (HEK) 293T cells and its acetylation level was detected by an …

Jun 30, 2016 · In HEK293 cells stably expressing mCherry–TFEB (TFEB stable cells), we found that a mild increase in ROS levels due to CCCP treatment (5 μM for 1 h) was sufficient to …

Aug 3, 2020 · Overexpression of GFP–GABARAP increased TFEB nuclear translocation in HEK293T (293T) cells but not in cells stably expressing RagB Q99L (Fig. 4l; Extended Data …

Dec 17, 2023 · To explore the mechanism of Erbin-regulated TFEB cytoplasm-to-nucleus translocation, we transfected with HA-Erbin and Flag-TFEB in 293T cells, immunoprecipitation …

However, co-transfection of Sox2 and TFEB significantly enhanced the luciferase activity of the reporter in HEK 293T cells (Fig. 3a). Sox2 is known to form a heterodimer with Oct4 to activate …

Nov 11, 2023 · We overexpressed wild-type and mutant Flag-TFEB in 293T cells, and then enriched TFEB protein by immunoprecipitation (Fig. 6A). Through mass spectrometry analysis, …

Sep 19, 2024 · TFEB/TFE3 activation via the channel of STING integrates cytosolic DNA sensing, a general cellular stress-sensing mechanism, with lysosomal biogenesis and autophagy …

Feb 2, 2013 · Active Rag GTPases are required for recruitment of TFEB to lysosomes and its phosphorylation by mTORC1, inhibiting its function under nutrient-rich conditions.

To assess the ability of TFEB-L and TFEB-S to promote autophagy, 293T cells stably expressing TFEB-L and TFEB-S were transfected with an LC3 expression vector fused to GFP. …

Feb 17, 2012 · TFEB subcellular localization was analysed in HeLa and HEK‐293T cells transiently transfected with a TFEB–3 × FLAG plasmid and treated overnight with several …