TAp63-mediated senescence overrides Ras-driven transformation of p53-deficient cells, preventing tumour initiation, and doxycycline-regulated expression of TAp63 activates p21 Waf/Cip1, induces senescence and inhibits progression of established tumours in vivo.

We found that downregulation of p53, p63 and TAp63 decreases the expression of growth-suppressing genes, including p21, PUMA and MIC-1. Interestingly, downregulation of p63, particularly TAp63, but not p53, disrupts the ability of MDCK cells to form a cyst structure in 3-D culture via heightened epithelial-to-mesenchymal transition (EMT).

All isoforms of TAp63 transactivate the p21-encoding gene, with TAp63β showing the highest levels of p21 induction (Helton et al., 2008). The transcriptional activation of p21 by TAp63 requires both the AD and proline-rich domains of TAp63.

Apr 1, 2013 · We found that like downregulation of p53, downregulation of p63 and TAp63 decreases expression of growth-suppressing genes, including p21, PUMA and MIC-1, and consequently promotes cell...

Sep 11, 2020 · All isoforms of TAp63 transactivate the p21-encoding gene, with TAp63β showing the highest levels of p21 induction (Helton et al., 2008). The transcriptional activation of p21 by TAp63 requires both the AD and proline-rich domains of TAp63.

May 1, 2008 · The TAp63 variant requires both the activation domain and the proline-rich domain to transactivate target gene promoters. (a) The ability of p63 isoforms to regulate the P21, FDXR, and GADD45 promoters was measured by luciferase reporter assay.

Nov 21, 2013 · In this report, we demonstrate that TAp63 + TECs colocalize with senescence marker p21 and correlate with an increase in senescent cell clusters in the naturally (wild-type, WT) aged murine thymi.

Nov 8, 2009 · TAp63-mediated senescence overrides Ras-driven transformation of p53-deficient cells, preventing tumour initiation, and doxycycline-regulated expression of TAp63 activates p21 Waf/Cip1,...

SCF TrCP1 is able to bind p63 isoforms, with a higher affinity for the TAp63 isoform. Strikingly, co-expres-sion of TAp63 and TrCP1 leads to the stabilization of TAp63 . This stabilization of TAp63 leads to up-regulation of p21 at the mRNA and protein level by increased binding of TAp63 at the p21 promoter. The up-regulation of p21 causes a.

Thus, TAp63 has an activity similar to p53, such as in inducing p21 [3]. ΔNp63 lacks the activation domain conserved in p53 but carries a unique activation domain in the N-terminus, which is composed of 14 unique N-terminal residues and the proline-rich domain [4].