Dec 27, 2019 · MSC-osteoblasts differentiated for 21 days displayed about 50% higher phosphorylation levels of p70-S6 kinase at threonine 389 (p70-S6K; Fig. 3A), a direct downstream target of mTORC1 (Fig. 3I,J).

Phospho-p70 S6 Kinase (Thr389) (1A5) Mouse mAb detects endogenous levels of p70 S6 kinase only when phosphorylated at Thr389. This antibody also detects p85 S6 kinase when phosphorylated at the analogous site (Thr412) and possibly S6KII phosphorylated at Thr388. Human, Mouse, Rat, Monkey, D. melanogaster.

Oct 31, 2003 · p70 S6K was found to be constitutively phosphorylated in the prostate cancer cells by the addition of serum. The activation of p70 S6K is accompanied by the phosphorylation at multiple Ser/Thr residues such as Ser389 and Ser421/Thr424.

Jul 7, 2020 · In the present study, we showed that ULK1 phosphorylated Ser389 of PDK1 at the linker region. This phosphorylation did not affect the kinase activity of PDK1. However, the nonphosphorylatable mutant of PDK1 showed weak activity toward the downstream kinases, such as Akt and p70S6k.

Oct 15, 2002 · Exposure of cells to UV radiation led to marked increases in p70 S6k activity and phosphorylation at Thr 389 and Thr 421 /Ser 424. UV radiation also generated reactive oxygen species as measured by electron spin resonance and by H 2 O 2 and O ⨪ 2 fluorescence staining assays in JB6 Cl 41 cells.

Nov 18, 2005 · We show that mTOR and S6K1 maneuver on and off the eukaryotic initiation factor 3 (eIF3) translation initiation complex in a signal-dependent, choreographed fashion. When inactive, S6K1 associates with the eIF3 complex, while the S6K1 activator mTOR/raptor does not.

Sep 18, 2021 · The results obtained above supported that both F1 and F3 inhibited the expression of p-Akt (Ser473), p-Akt (Thr308), p-GSK-3β (Ser9), p-mTOR (Ser2448), and p-p70 S6K (Ser389), whereas the expression of Akt, GSK-3β, mTOR, and p70 S6K had no significant change in Jurkat cells.

Nov 1, 2006 · IL-2-stimulated CTLL-2 cells were cultured in the presence of 100 nM BraA or rapamycin for 18 hr, and the phosphorylation of the substrates of mTOR (p70 S6k on Ser389) and GCN2 (eIF2α on Ser51) was investigated.

Feb 11, 2020 · The immunosuppressant drug rapamycin and its analogs that inhibit mTOR are currently being evaluated for their potential as anti-cancer agents, albeit with limited efficacy. mTORC1 mediates its function via its downstream targets 40S ribosomal S6 kinases (S6K) and eukaryotic translation initiation factor 4E (eIF4E)-binding protein 1 (4E-BP1).

Feb 5, 2018 · Here, we show that the mechanistic target of rapamycin (mTOR)–ribosomal S6 kinase (S6K) pathway, a central regulator of growth signalling, phosphorylates RNF168 at Ser60 to inhibit its E3 ligase...