The ATP-dependent activities of the Rad50 protein are essential for all of its roles in DNA repair and signaling, as mutations of residues involved in ATP binding and hydrolysis exhibit phenotypes equivalent to a rad50 deletion in vivo (41–43).

Rad50 is a member of the structural maintenance of chromosomes (SMC) family of proteins. [6] Like other SMC proteins, Rad50 contains a long internal coiled-coil domain that folds back on itself, bringing the N- and C-termini together to form a globular ABC ATPase head domain.

Aug 13, 2018 · The rad50-C1G and rad50-C2G mutants retained partial resistance to several DNA damaging agents, including camptothecin, hydroxyurea, and ionizing radiation (IR) (Fig. 5B). These sensitivities might be partly explained by Rad50 instability, as immunoblotting revealed substantial reductions of Rad50 in both mutants ( Fig. 5 C ).

Rad50 resembles the SMC proteins involved in chromosome cohesion and chromatin condensation (Aravind et al. 1999; Strunnikov and Jessberger 1999). SMC proteins all contain Walker A and B nucleotide (NTP)-binding motifs at their amino- …

Mar 4, 2025 · The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends.

Jun 23, 2000 · To clarify the key role of Rad50 in DNA double-strand break repair (DSBR), we biochemically and structurally characterized ATP-bound and ATP-free Rad50 catalytic domain (Rad50cd) from Pyrococcus furiosus. Rad50cd displays ATPase activity plus ATP-controlled dimerization and DNA binding activities.

Dec 25, 2024 · RAD50 (RAD50 Double Strand Break Repair Protein) is a Protein Coding gene. Diseases associated with RAD50 include Nijmegen Breakage Syndrome-Like Disorder and Hereditary Breast Ovarian Cancer Syndrome.

In our study, RAD50 germline mutations were determined using next-generation sequencing in 7657 consecutive unselected breast cancer patients without BRCA1/2 mutations. We also screened for RAD50 recurrent mutations (L719fs, K994fs, and H1269fs) in 5000 healthy controls using Sanger sequencing.

RAD50 - Explore an overview of RAD50, with a histogram displaying coding mutations, full tabulated details of all associated variants, tissue distribution and any drug resistance data.

RAD50 homolog (RAD50) is a gene that encodes a protein that functions in double-strand break repair. Missense mutations, nonsense mutations, silent mutations, and frameshift deletions and insertions are observed in cancers such as endometrial cancer, …