Oct 31, 2002 · RACK1 is one of a group of PKC-interacting proteins collectively called RACKs (Receptors for Activated C-Kinases). Previously, we showed that RACK1 also interacts with the Src tyrosine kinase, and is an inhibitor of Src activity and cell growth.

This enhanced PKC beta II activation is coupled with increased RACK1 expression and PKC beta II-RACK1 interactions, demonstrating PKC epsilon-induced PKC beta II signaling via a RACK1-dependent mechanism.

Aug 5, 2005 · Here, we demonstrate that the receptor for activated C kinase 1 (RACK1) serves as an adaptor for PKC-mediated JNK activation. Phosphorylation of JNK by PKC occurs on Ser129 and requires the presence of RACK1.

Jan 31, 2025 · Regulation of PKC activities has also been shown to be exerted by a family of proteins called receptors for activated C kinase (RACK) which are a family of membrane-associated PKC anchoring proteins. RACK serve as molecular scaffolds that localize individual PKC to distinct membrane microdomains in close proximity to their allosteric activators ...

Oct 17, 2001 · Here, we describe the role of adaptor proteins in determining the specific function of individual protein kinase C (PKC) isozymes. These isozyme-selective proteins were called collectively...

In summary, we have shown that β′-COP fulfills the criteria for a PKCε-selective RACK: it binds activated PKCε better than any other isozyme, and PKC binding is dose-dependent and saturable. β′-COP co-immunoprecipitates and colocalizes with …

May 1, 1991 · Binding of PKC to two of these proteins was concentration-dependent, saturable, and specific, suggesting that these binding proteins are receptors for activated C-kinase, termed here "RACKs." PKC binds to RACKs via a site on PKC distinct from the substrate binding site.

Sep 1, 1997 · Recent studies have clarified the key role played by the anchoring proteins in mediating subcellular PKC location, that is, in driving the enzyme to specific sites of action. The protein, receptor for activated C-kinase 1 (RACK1) is involved in …

Protein scaffolds such as A-kinase anchoring proteins (AKAPs) and receptors for activated C-kinase (RACKs) organize PKCs, their substrates, and their modulators into subcellular complexes that coordinate signaling.

C. PKC Interactions With RACK Proteins. Models of PKC activation have generally focused on the intramolecular interaction between the pseudosubstrate domain and the catalytic pocket; PKC mutations that disrupt this intramolecular interaction (such as a pseudosubstrate domain deletion or an alanine→glutamate phosphomimetic substitution in the ...