Essentially, Prism uses the row number as the X value, so the points will be equally spaced along the X axis, even if the X values are not evenly spaced. By default, each row label will appear. If you have many rows, this will be cluttered.

To create a discontinuous axis with Prism requires three steps. Double-click on the axis to open the Format Axes dialog. In the Gaps and Directions section, you can choose either a two-segment (one gap) or three-segment (two gaps) axis.

Prism can also plot horizontal X error bars. You must enter X error values directly; Prism cannot compute them. You can only enter one value for X errors, so X error bars are always symmetrical. Entering multiple sets of data that don't share X values. Each data table has a single column for X and up to 104 sets of Y values.

On an XY or column graph, when the Y axis spans zero, Prism automatically places a grid line at Y=0. This is easy to remove. Double click on the axis, to bring up Format Axis dialog.

The Rotate/Flip button in the Change section of the Prism toolbar drops a menu with three choices. You can reverse the order of data sets on a graph, flip the page settings between landscape or portrait orientation, or rotate column graphs from vertical to horizontal

Prism's default is to add the Y axis title to the left of the axis, but you can choose a different default location in Prism's preferences. Additionally, Prism will automatically choose an appropriate rotation for the text (Vertical (Up), Vertical (Down), or …

The preferred analysis and graphing solution purpose-built for scientific research. Join the world’s leading scientists and discover how you can use Prism to save time, make more appropriate analysis choices, and elegantly graph and present your scientific research.

Two X axes . How to do it: Prism does not allow a single graph to have two X axes or three Y axes. You can, however, create that effect with a bit of a workaround. Create two graphs. On one, set the origin of the graph to be the upper right, To do this double-click on either axis and go to the Frame and Origin tab of the Format Graph dialog.

Graph tip -- How to make an XY graph with a time-scale on the X axis? This example shows several tips: how to use time (or anything non-numerical) as axis labels, how to create custom ticks, how to shade portions of a graph, and how to add …

GraphPad Prism does not really let you choose the style (solid, dashed, dotted) of the axes, but if you are a bit tricky you can make it happen. Follow these steps. Go to the first tab of Format Axis, and choose to hide the axis that you want to be dotted or dashed.