PstI is a type II restriction endonuclease isolated from the Gram negative species, Providencia stuartii. PstI cleaves DNA at the recognition sequence 5′-CTGCA/G-3′ generating fragments with 3′-cohesive termini. [1] . This cleavage yields sticky ends 4 base pairs long. PstI is catalytically active as a dimer.

PstI has a High Fidelity version PstI-HF ® (NEB #R3140). High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity.

Thermo Scientific PstI restriction enzyme recognizes CTGCA^G sites and cuts best at 37°C in O buffer (Isoschizomers: BspMAI). See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and …

PstI-HF has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10227210. Learn more. We are excited to announce that all reaction buffers are now BSA-free.

Pst I is a type II restriction enzyme that is commonly used in molecular biology research to manipulate DNA fragments. It is derived from the bacterium Providencia stuartii and cleaves DNA at the recognition site 5′-CTGCA/G-3′ to generate DNA fragments with 3′-cohesive termini.

Mar 1, 2021 · PstI is a restiction enzyme used in molecular biology applications to cleave DNA at the recognition sequence 5′-CTGCA/G-3′, generating DNA fragments with 3′- cohesive termini.

Thermo Scientific FastDigest PstI is one of an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers.

Pst I endonuclease was detected in osmotic shock fluid from each of the resistant clones. Plasmid and chromosomal DNA from these clones could not be digested by Pst I, indicating that the gene for the corresponding modification enzyme had also been cloned and was being expressed.

Pst I, derived from the bacterial strain Providencia stuartii, is a Type II restriction endonuclease. As a homodimeric enzyme, Pst I recognizes a specific DNA sequence known as the recognition site or restriction site.

Sigma-Aldrich offers Roche-PSTI-RO, Pst I Restriction Enzyme solution from Providencia stuartii for your research needs. Find product specific information including CAS, MSDS, protocols and references.