
Determination of the Specific Substrate Sequence Motifs of …
In this study, we have determined the optimal peptide substrate sequence for each of nine human PKC isozymes (α, βI, βII, γ, δ, ϵ, η, μ, and ζ) by using an oriented peptide library.
mTORC2 Controls PKC and Akt by Phosphorylating a Conserved …
mTORC2 binds and phosphorylates a conserved TOR-Interaction Motif in the C-tail of PKC and Akt to regulate hydrophobic motif autophosphorylation and kinase activation. Main Text:
Protein kinase C - Wikipedia
The conventional and novel PKCs have three phosphorylation sites, termed: the activation loop, the turn motif, and the hydrophobic motif. The atypical PKCs are phosphorylated only on the activation loop and the turn motif.
Regulation of Protein Kinase C function by phosphorylation on ... - PubMed
Protein Kinase C (PKC) is a family of serine/threonine kinases whose function is influenced by phosphorylation. In particular, three conserved phosphorylation sites known as the activation-loop, the turn-motif and the hydrophobic-motif play important roles in …
mTORC2 controls the activity of PKC and Akt by phosphorylating a ...
Baffi et al. identified a distinct and evolutionarily conserved motif in Akt and PKC termed the TOR interaction motif (TIM) whose phosphorylation by mTORC2 was required for catalytic competence.
Protein kinase C and beyond - Nature Immunology
Jul 28, 2004 · The classical PKC isoforms (cPKC) share all typical regulatory features: the autoinhibitory pseudosubstrate motif, two DAG-binding C1 domains (C1a and C1b) and the calcium-binding C2 domain.
mTORC2 controls the activity of PKC and Akt by phosphorylating a ...
Apr 13, 2021 · Overexpression of PDK1 in mTORC2-deficient cells rescued hydrophobic motif phosphorylation of PKC and Akt by a mechanism dependent on their intrinsic catalytic activity, revealing that mTORC2 facilitates the PDK1 phosphorylation step, which, in turn, enables autophosphorylation.
PTMScan® Phospho-PKC Substrate Motif [(R…
PTMScan ® Phospho-PKC Substrate Motif [(R/K)(R/K)Xp(S/T)X(R/X)] Kit #71652 Additional Information This product is intended for peptide enrichment and mass spectrometry analysis.
Protein kinase C (PKC) isozyme-specific substrates and their design
Nov 1, 2012 · Protein kinase C (PKC), a phospholipid-dependent serine/threonine kinase, appears to be involved in the signal transduction response to many hormones and growth factors; there are 11 different PKC isozymes.
Phospho-PKC Substrate Motif [ (R/K)XpSX (R/K)] MultiMab
Phospho-PKC Substrate Motif [(R/K)XpSX(R/K)] MultiMab ® Rabbit mAb mix detects endogenous levels of many cellular proteins only when phosphorylated at Ser residues surrounded by Arg or Lys at the -2 and +2 positions. The antibody may also …