Western blot analysis of extracts from SW-13, NIH/3T3, Jurkat and PC12 cells, using PDK1 Antibody. Cat. Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody. PDK1 Antibody detects endogenous levels of total PDK1 protein.

10026-1-AP targets PDK1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples. PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for …

Jun 6, 2023 · Monomeric full-length PDK1 1-556 had D max = 110 Å and Rg = 34.8 Å, with the MW estimated between 67 and 79 kDa by different calculation approaches (Table 1).

Phospho-PDK1 (Ser241) Antibody is a rabbit antibody raised against a short peptide from N human PDK1 sequence surrounding and containing phospho-Ser241. This antibody detects endogenous phosphorylated PDK1 (Ser241) proteins …

Recombinant full-length house PDK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. The gene accession number is NM_002613. Figure 1. SDS-PAGE gel image. The purity of PDK1 was determined to be >85% by densitometry. Approx. MW 67kDa.

Dec 25, 2024 · PDK1 (Pyruvate Dehydrogenase Kinase 1) is a Protein Coding gene. Diseases associated with PDK1 include Pyruvate Dehydrogenase E3-Binding Protein Deficiency and Polycystic Kidney Disease 1 With Or Without Polycystic Liver Disease .

3-Phosphoinositide-dependent kinase 1 (PDK1), also known as PKB kinase, was identified as the activator of the survival kinase Akt/PKB. Several important substrates of PDK1 include p70S6 kinase, PKAs, PKCs, SGKs, RSKs, and PAKs. PDK1 is a member of the AGC superfamily of serine/threonine kinases.

We provide genetic evidence that PDK1 is essential for mouse embryonic development, and regulates cell size independently of cell number or proliferation, as well as insulin’s ability to activate PKB, S6K and RSK. Keywords: cell growth/cell size/embryonic development/PDK1/PKB–Akt/PI 3-kinase.

PDK1 mRNA expression is significantly associated with tumor progression; in fact, the presence of PDK1 can serve as a prognostic marker, indicating the level of success a patient can achieve. Specifically, this may serve as a biomarker in patients with gastric cancer.

Polyclonal Antibody for studying PDK1 (Ser241) phosphate. Validated for IP, WB. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-PDK1 (Ser241) Antibody (CST #3061) is ready to ship.