PHLs are now using NGS applications for foodborne pathogens, infectious disease and, in some cases, newborn screening. This technology allows for greater information about pathogens leading to more informed outbreak and surveillance studies.

Fundamental to NGS library construction is the preparation of the nucleic acid target, RNA or DNA, into a form that is compatible with the sequencing system to be used (Figure 1). Here, we compare and contrast various library preparation strategies and NGS applications, focusing primarily on those compatible with Illumina sequencing technology.

Mar 14, 2017 · To ensure clinical grade quality without inhibiting adoption of NGS, a taskforce team assembled by the Korean Society of Pathologists developed laboratory guidelines for NGS cancer panel testing procedures and requirements for clinical implementation of NGS.

We present here recommendations for the design, optimization and implementation of an informatics pipeline for clinical next-generation sequencing (NGS) to detect germline sequence variants in compliance with existing regulatory and professional quality standards 1.

These recommendations and best practices are designed to help you ensure the success of your next-generation sequencing (NGS) experiment. Review key considerations, such as calculating read length, estimating coverage, and measuring sequencing accuracy.

Nov 6, 2024 · We can prepare standard as well as specialized libraries of various types, including genomic DNA with different size inserts, RNA-seq with Ribo-depletion or strand specific options, exome capture, ChIP-seq, and microRNA-seq.

Jan 1, 2014 · Next-generation sequencing (NGS, also called second-generation (G2) sequencing) is so named relative to Sanger or first generation (G1) sequencing. NGS has three characteristics: (1) A need for clonal amplification of templates; (2) Sequencing is undertaken in a massively parallel way; and (3) Short read lengths (<700 bp) are generated .

The design of the second-generation sequencing NGS laboratory is divided into four areas, namely: reagent preparation area, specimen and library preparation area, library amplification and detection area, and sequencing area.

The Introduction to NGS and Next-generation sequencing for beginners pages are great places to start for learning about Illumina sequencing and accessing supporting resources. The [Sequencing: How to Plan Your First Sequencing Project] (

In principle, NGS laboratories should be divided into zones: sample pre-processing area, reagent storage and preparation area, sample preparation area, library preparation area, hybrid capture area/multiplex PCR area (first amplification area), library amplification area (Second amplification area), library detection and quality control area ...