The use of the Cre/loxP system for generating tissue specific or inducible knockouts is a powerful tool for mouse genetics, but it is only one piece of the total picture. Below, I would to focus on …

May 18, 2006 · The FLP-FRT system. The FLP-FRT system is similar to the Cre-lox system and is becoming more frequently used in mouse-based research. It involves using flippase (FLP) …

The Cre-lox system is one of the most revolutionary tools in the geneticist's toolbox. You don't have to be a user or developer of cre or floxed mice to appreciate the impact that cre-lox …

Conditional mutagenesis using the Cre-lox system is not the only strategy available to spatiotemporally regulate gene expression in mouse models of human diseases. New …

The Cre/lox system can also be used to produce strains in which a transgene is either inducible or expressed only in certain tissues. For example, mating the transgenic strain in Figure 1 to a …

The simplest Cre-lox system to generate a knockout (KO) mouse model has two components that need to be genetically engineered in the murine genome: a target sequence surrounded by …

The Cre/lox system has become the cornerstone of modern mouse genetics because it enables sophisticated control over the timing and location of gene expression. This is the first in a …

Sep 22, 2011 · More novel and sophisticated uses for the Cre/lox system are being developed all the time. Research mouse models that incorporate the Cre/lox system typically involve …

A. Research Tools: Cre-Lox and More FAQ. How do Cre-Lox and Tet-on / Tet-off systems work? Click here for an introduction to Tet Expression Systems. Click here for an on demand webinar …

With tissue-specific Cre-lox KO models, the genotype of the mice that you generally want to evaluate ishomozygous for your floxed allele (fl/fl) and positive for Cre (cre+). (Typically, only …