Sep 10, 2018 · The authors reported that “resting HMC3 cells were strongly positive for the microglia/macrophage marker IBA1 (Fig. 1a), positive for the endotoxin receptor CD14 (Fig. 1b), but negative for the astrocyte marker GFAP (Fig. 1c and d).

The effect of AME-1 on HMC3 production of IL-8 and IL-6 was determined and compared to tumor necrosis factor (TNF), polyinosinic-polycytidylic acid [poly(I:C)], substance P and...

Resting HMC3 cells were strongly positive for the microglia/macrophage marker IBA1, positive for the endotoxin receptor CD14, but negative for the astrocyte marker GFAP. Markers of activated microglia, namely MHCII, CD68 and CD11b were negative in resting HMC3 cells, but upregulated after activation by IFN-gamma (10 ng/ml, 24 h).

HMC-3 cells immuno-labeled for IBA1 (green fluorescence, d, f) and DAPI stained (blue fluorescence, a, b) are shown. Merged images are shown in (e, f). Control experiments performed by omitting...

Sep 10, 2018 · HMC-3 cells immuno-labeled for IBA1 (green fluorescence, d, f) and DAPI stained (blue fluorescence, a, b) are shown. Merged images are shown in (e, f).

Apr 23, 2021 · In this study, we investigated the mechanisms underlying the anti-inflammatory and anti-migratory functions of JQ1 and the genes targeted by JQ1 in lipopolysaccharide (LPS)-activated human...

IFNγ and TNFα enhanced expression of Iba1. HMC-3 cells express higher level of TLR9 than TLR4. Conclusions: IFNγ and TNFα induce activation of HMC-3 triggering NF-κB, ERK1/2 and p38 pathways. Activation of HMC-3 cells with TNFα leads to greater inflammatory response than IFNγ stimulation.

Dec 31, 2024 · The HMC3 cell line was established through SV40-dependent immortalization of primary microglia cultures derived from human fetal brain tissue. In resting HMC3 cells, the microglia/macrophage marker IBA1 is strongly positive, while the …

Sep 10, 2018 · The human microglial clone 3 cell line, HMC3, was established in 1995, through SV40-dependent immortalization of human embryonic microglial cells. It has been recently authenticated by the American Type Culture Collection (ATCC®) and distributed under the name of HMC3 (ATCC®CRL-3304).

Jul 1, 2018 · Changes in TREM2 and the microglial marker IBA1 in Aβ treated HMC3 cells, compared to untreated cells, were analyzed by immunofluorescence. Changes in levels of some of these key AD-related proteins; ADAM10, apoE, TREM2 and sTREM2, were observed upon Aβ treatment, compared to untreated HMC3 microglial cells.