H-89, Dihydrochloride A cell-permeable, selective, reversible, ATP-competitive and potent inhibitor of protein kinase A (Ki = 48 nM).; CAS Number: 127243-85-0; Synonyms: H-89, Dihydrochloride,N-[2-(( p-Bromocinnamyl)amino)ethyl]-5-isoquinolinesulfonamide, 2HCl, PKA Inhibitor III,N-[2-((p-Bromocinnamyl)amino)ethyl]-5-isoquinolinesulfonamide ...

H-89 dihydrochloride hydrate is a selective inhibitor of protein kinase A (PKA). It also inhibits potassium (K +) current in rat myocytes. It mediates Na + transport by interacting with α subunits of epithelial Na + channel (ENaC).

H-89 is a cell-permeable selective and potent inhibitor of protein kinase A (K i = 48 nM). Inhibits other kinases at several fold higher concentrations: myosin light chain kinase (K i = 28.3 µM), Ca 2+ /calmodulin-dependent protein kinase II (K i = 29.7 µM), protein kinase C (K i = 31.7 µM), casein kinase I (K i = 38.3 µM), and Rho Kinase ...

H-89 is a potent, cell permeable inhibitor of PKA that demonstrates an IC 50 value of 0.14 µM and a K i value of 48 nM in standard kinase assays. 1,2 While widely used to disrupt PKA signaling, the inhibitory activity of H-89 is non-selective. H-89 also inhibits S6K1, MSK1, ROCK2, PKBα, and MAPKAP-K1b with IC 50 values of 0.08, 0.12, 0.27, 2. ...

H 89 2HCl is a potent PKA inhibitor. In a cell-free assay, the Ki of H 89 is 48 nM, 10-fold selective for PKA than PKG and 500-fold greater selectivity than PKC, MLCK, calmodulin kinase II and casein kinase I/II [1].

H 89 2HCl is a potent PKA inhibitor with K i of 48 nM in a cell-free assay, 10-fold selective for PKA than PKG,500-fold greater selectivity than PKC, MLCK, calmodulin kinase II and casein kinase I/II.

H-89 is a potent selective inhibitor of cAMP dependent protein kinase (PKA). The in vitro IC50 of H-89 for PKA is approximately 50 nM and in vivo the inhibitiory effect on PKA substrate phosphorylation and related cellular functions range from 10 μM to 30 μM (1,2).