Jun 19, 2020 · GL261 glioblastoma cells were injected slowly to a depth of 3 mm ventral at 3 ×10 3 per µl in a volume of 5 µl over 2 min using a 10-µl Hamilton syringe connected to a pump by …

Feb 8, 2016 · First, we confirmed that EGFP-positive cells of the GL261/CX3CR1 glioma model express Iba1 (Fig. 3a–c). Iba1 is a marker of microglia/macrophages and can also indicate the …

Aug 25, 2011 · We demonstrated that Iba1-positive cells infiltrate implanted GL261 gliomas and close distance interactions result in amoeboid transformation of these cells.

Jan 27, 2012 · We found that microglia stimulate the invasion of GL261 glioblastoma cells by approximately eightfold in an in vitro invasion assay. Pharmacological inhibition of epidermal …

In this study, using a Gl261-C57Bl/6 mouse glioma implantation model, we identified significant upregulation of proline-rich tyrosine kinase Pyk2 and focal adhesion kinase (FAK) …

May 19, 2016 · To address the cellular basis and dynamics of the inflammatory microenvironment in GBM, we established an orthotopic syngenic model by grafting GL261-DsRed cells in …

Aug 8, 2023 · Indeed, we found in the GL261 model that combining Evo and irradiation eradicated hypoxic niches, abolished TAM sequestration, and achieved better tumor control.

Mouse brains injected with GL261 glioma cells expressing EGFP (green) were studied for the microglia marker Iba1 (blue) and for the metalloprotease MT1-MMP (red).

6: Histological analysis of mouse brains carrying GL261 tumors treated with AAV9/P2Int-mIFN Representative brain sections immune-stained with antibodies to (A) Iba1 or (B) CD3 to detect …

Jan 31, 2018 · Our findings show how manipulating Nrp1 in either peripheral macrophages or microglia reprograms their phenotype and their pathogenic roles in tumor neovascularization …