Dec 1, 2006 · GFP fusion proteins can be transiently or stably expressed. Although transient expression is quick and can provide informative results, in many cases it is beneficial and/or …

In this unit, strategies and background for designing and creating fluorescent fusion proteins (FFPs) are described.

The fusion of fluorescent tags to proteins in a host cell is a widely popular technique used in experimental cell and biology research in order to track protein interactions in real time. The …

In this blog I discuss strategies to generate fusion proteins and highlight some aspects of their design. The green fluorescent protein (GFP) from Aequorea victoria and its variants are …

Once, GFP fusion proteins from a collaborator produced unexpectedly rapid protein mobilities in cells. Sequencing revealed the GFP contained the three EGFP mutations and two additional …

Aug 23, 2024 · In order to generate a GFP fusion protein, a cDNA that encodes the protein of interest must be available or obtainable. Then, through molecular cloning, the cDNA for GFP …

GFP can be detected in living cells without selection or staining and it can be fused to other proteins to yield fluorescent chimeras. The potential of GFP has also been recognised by gene …

In the current study, we developed an improved purification method, utilizing a FMU-GFP.5 monoclonal antibody (mAb) to GFP together with a mAb-coupled affinity chromatography …

Aug 31, 1999 · Fluorescence correlation microscopy (FCM) was applied to characterize fusion proteins of the green fluorescent protein (GFP) on the cellular as well as molecular level within …

Here we describe a transposon-based approach for rapidly creating libraries of GFP fusion proteins. We tested our approach on the glutamate receptor subunit, GluR1, and the G protein …