Fluorescence in situ hybridization (FISH) is a powerful tool used in karyotyping, cytogenotyping, cancer diagnosis, species specification, and gene expression analysis. Although widely used, conventional FISH protocols are cumbersome and time ...

HeLa (/ ˈ h iː l ɑː /) is an immortalized cell line used in scientific research. It is the oldest human cell line and one of the most commonly used. [1] [2] HeLa cells are durable and prolific, allowing for extensive applications in scientific study.

A combination of these techniques [comparative genomic hybridization (CGH), fluorescence in situ hybridization (FISH), and spectral karyotyping (SKY)] has been used to determine the karyotype of a CCL2 HeLa cell line (Macville et al. 1999).

Oct 26, 2018 · Fluorescence in situ hybridization (FISH) is the primary technology used to image and count mRNA in single cells, but applications of the technique are limited by photophysical shortcomings of ...

By using fluorescent in situ hybridization (FISH), we visualized viral RNA of human rhinovirus type 2 (HRV2) during its entry into HeLa cells. RNA uncoating of HRV2 is entirely dependent on low endosomal pH (≤5.6).

Oct 25, 2021 · Fluorescence in situ hybridization (FISH) is a powerful tool to visualize transcripts in fixed cells and tissues. Despite the recent advances in FISH detection methods, it remains challenging to ...

Comparative data about distribution of this number among individual acrocentric chromosomes have been obtained by FISH with rDNA probes on the preparations of spread mitotic chromosomes, for the ...

RNA-FISH is a powerful method to detect specific RNAs in fixed cells. It allows both localization and quantification of RNA molecules within individual cells and tissues. Refined RNA-FISH methods have also been developed to determine RNA transcription and degradation rates.

May 16, 2023 · Fish is an excellent choice for any menu since it has a quick cook time and is super nutritious. When searching for new methods to flavour your fish dishes, plenty of spices and herbs are available to elevate the flavours. Many provide a mild taste without overpowering the plate, while others create an exciting punch.

Fluorescence in situ Hybridization (FISH) utilizes peptide nucleic acid (PNA) probes to identify specific DNA sequences. PNA probes have been effectively used to identify chromosome aberrations and have been shown to greatly aid in biodosimetery assays involved in …