pcDNA3.1(-) FMC-63 scFv-Fc (huFc) - Addgene
Plasmid pcDNA3.1 (-) FMC-63 scFv-Fc (huFc) from Dr. Alexander McLellan's lab contains the insert anti-CD19 scFvFc (FMC-63). This plasmid is available through Addgene.
RCSB PDB - 7URV: FMC63 scFv in complex with soluble CD19
Apr 22, 2022 · We report cryo-EM structures of CD19 antigen with the binder FMC63, which is used in four FDA-approved CAR T cell therapies (Kymriah, Yescarta, Tecartus, and Breyanzi), …
Solving the mystery of the FMC63-CD19 affinity
Dec 27, 2023 · In a first experimental system, we titrated the FMC63-scFv on the human CD19 + leukemia model cell line NALM6 using various incubation times, as well as different conditions …
Solving the mystery of the FMC63-CD19 affinity - PubMed
Dec 27, 2023 · The majority of approved CAR T cell products are based on the FMC63-scFv directed against CD19. Surprisingly, although antigen binding affinity is a major determinant …
To minimize these artefacts, we performed SPR with strictly monomeric and correctly folded soluble CD19, yielding an FMC63‐scFv afinity of 2–6 nM. Together, apart from analyzing the …
Identification of Potent CD19 scFv for CAR T Cells through scFv ...
Most of these anti-CD19 CAR T cells use FMC63 single-chain variable fragments (scFvs) for binding CD19 expressed on the cancer cell surface. In this study, we screened several known …
Design of sensitive monospecific and bispecific synthetic chimeric …
1 day ago · The CD19-specific FMC63 scFv and the CD22-specific 9A8 scFv were used for the Bi-ChTCR; FMC63 and m971 scFvs were used in all CARs. Image created using …
In silico and in vivo analysis reveal impact of c-Myc tag in FMC63 scFv …
Dec 1, 2024 · Here, we conducted in-silico structural modelling of an FMC63-based anti-CD19 single-chain variable fragment (scFv) with and without a c-Myc peptide tag added to the N …
Potent anti-leukemia activities of humanized CD19-targeted ... - PubMed
The immune response induced by murine single-chain variable fragment (scFv) of the CAR may limit CAR-T cell persistence and thus increases the risk of leukemia relapse. In this study, we …
Identification of Potent CD19 scFv for CAR T Cells through scFv ...
Dec 1, 2020 · Most of these anti-CD19 CAR T cells use FMC63 single-chain variable fragments (scFvs) for binding CD19 expressed on the cancer cell surface. In this study, we screened …