The present study demonstrates important effects of DTT on DNA. Using standard molecular biological reaction conditions, we show that DTT is able to introduce single stranded nicks in double stranded DNA. Furthermore, DTT was able to facilitate immobilization of fluorescently labelled DNA to a functionalized microscopy slide.

DTT is used as a reducing or "deprotecting" agent for thiolated DNA. The terminal sulfur atoms of thiolated DNA have a tendency to form dimers in solution, especially in the presence of oxygen. Dimerization greatly lowers the efficiency of subsequent coupling reactions such as DNA immobilization on gold in biosensors.

May 24, 2017 · Using two different assays that can sense strand interruptions in double stranded DNA, we here show that DTT is able to introduce nicks in the DNA backbone. DTT was furthermore shown to facilitate the immobilization of fluorescent DNA on an NHS-ester functionalized glass surface.

May 24, 2017 · Motivated by the emergence of single molecule detection methods and the presence of DTT in virtually all traditionally used DNA modification protocols, we set off to elucidate the effect of DTT on DNA and thereby its influence on the results of highly sensitive DNA based assays.

May 24, 2017 · Using two different assays that can sense strand interruptions in double stranded DNA, we here show that DTT is able to introduce nicks in the DNA backbone. DTT was furthermore shown to...

T4 DNA ligase is one of the most commonly used enzymes for _in vitro_ molecular research and a useful model for testing the ligation mechanism of ATP-dependent DNA ligation.

DTT acts in a surprisingly efficient manner when compared with “pure” UV-light based photo-cross-linking and thus can be actively used as a protein–RNA cross-linking enhancer in UV light induced cross-linking.

We aimed at testing both dithiothreitol (DTT) and enzymatic treatments of sputum samples and identifying the most suitable DNA extraction technique for the microbiota analysis of sputum.

DNA extractions of semen samples commonly utilize dithiothreitol (DTT) to reduce and disrupt disulfide bonds. Although traditional extraction techniques remove DTT before downstream analyses, the forensic DNA community has recently explored Y-screening, direct amplification, and direct cell lysis as …

DTT is a potent reducing agent widely exploited in molecular biology as an enzyme stabilizing agent and can be found in the supplied reaction buffers of many commercially available DNA...

Feb 2, 2024 · Ultraviolet A (UVA) radiation, present in sunlight, can induce cell redox imbalance leading to cellular damage and even cell death, compromising skin health. Here, we evaluated the in vitro antioxidant and photochemoprotective effect of dithiothreitol (DTT).

We have found that dithiothreitol (DTT) from the DNA extraction process can cause another type of real-time PCR disturbance, i.e., inhibition of signal detection through uorescence quenching.

Sep 1, 2012 · Protein reducing agents dithiothreitol (DTT) or tris (2-carboxyethyl)phosphine (TCEP) are crucial to disrupt disulfide bonds for qualitative and quantitative analysis in proteomics. Protein bond disruption is very important for analyzing proteins as single subunits.

DTT is used as a reducing or "deprotecting" agent for thiolated DNA. DTT is frequently used to reduce the disulfide bonds of proteins and, more generally, to prevent intramolecular and intermolecular disulfide bonds from forming between cysteine residues of proteins.

Sep 28, 2000 · Herein, we investigated the apoptotic pathways in HL-60 cells triggered by dithiothreitol (DTT), used as a model thiol compound, and tested the hypothesis that thiols cause apoptosis via...

Jan 31, 2018 · We here develop a single-step assay utilizing dithiothreitol (DTT) to fine-tune the surface coverage of DNA-modified AuNPs. DTT is superior to the commonly used surface diluent, mercaptohexanol, as it is less volatile, allowing for the rapid and reproducible controlling of surface coverage on AuNPs with only micromolar concentrations of DTT.

DTT quenches the fluorescence of the passive reference dye Mustang Purple in Quantifiler HP, causing overestimated DNA concentrations. Purification of DNA extracts containing DTT is needed to overcome the effect.

Dithiothreitol (DTT), also known as Cleland's reagent, is a small-molecule redox reagent. Its oxidized form is a disulfide-bonded 6-membered ring. It is used to break down protein disulfide bonds and stabilize enzymes and other proteins.

0.25 μM yeast or human CMG, Csm3-Tof1/TIM-TIPIN and Mrc1/ CLASPIN were incubated with 5 μM template DNA and 100 μM AMP-PNP for 30 min at 30°C (yeast proteins) or 37°C (human proteins) in a 20 ml reaction containing 20 mM HEPES-KOH pH 7.6/100 mM potassium acetate/10 mM magnesium acetate/2.5 mM DTT/0.02% NP40-S. DNA unwinding was initiated ...

Dithiothreitol [DTT] is also known as Cleland's Reagent. It contains two thiol groups and is commonly used to reduce disulfide bond in proteins. It can protect many thiol-containing biomolecules such as coenzyme A (Co-A), GSH (glutathione) from forming disulfide bonds.