The present study demonstrates important effects of DTT on DNA. Using standard molecular biological reaction conditions, we show that DTT is able to introduce single stranded nicks in …

DTT is used as a reducing or "deprotecting" agent for thiolated DNA. The terminal sulfur atoms of thiolated DNA have a tendency to form dimers in solution, especially in the presence of …

May 24, 2017 · Using two different assays that can sense strand interruptions in double stranded DNA, we here show that DTT is able to introduce nicks in the DNA backbone. DTT was …

May 24, 2017 · Motivated by the emergence of single molecule detection methods and the presence of DTT in virtually all traditionally used DNA modification protocols, we set off to …

May 24, 2017 · Using two different assays that can sense strand interruptions in double stranded DNA, we here show that DTT is able to introduce nicks in the DNA backbone. DTT was …

T4 DNA ligase is one of the most commonly used enzymes for _in vitro_ molecular research and a useful model for testing the ligation mechanism of ATP-dependent DNA ligation.

DTT acts in a surprisingly efficient manner when compared with “pure” UV-light based photo-cross-linking and thus can be actively used as a protein–RNA cross-linking enhancer in UV …

We aimed at testing both dithiothreitol (DTT) and enzymatic treatments of sputum samples and identifying the most suitable DNA extraction technique for the microbiota analysis of sputum.

DNA extractions of semen samples commonly utilize dithiothreitol (DTT) to reduce and disrupt disulfide bonds. Although traditional extraction techniques remove DTT before downstream …

DTT is a potent reducing agent widely exploited in molecular biology as an enzyme stabilizing agent and can be found in the supplied reaction buffers of many commercially available DNA...

Feb 2, 2024 · Ultraviolet A (UVA) radiation, present in sunlight, can induce cell redox imbalance leading to cellular damage and even cell death, compromising skin health. Here, we evaluated …

We have found that dithiothreitol (DTT) from the DNA extraction process can cause another type of real-time PCR disturbance, i.e., inhibition of signal detection through uorescence quenching.

Sep 1, 2012 · Protein reducing agents dithiothreitol (DTT) or tris (2-carboxyethyl)phosphine (TCEP) are crucial to disrupt disulfide bonds for qualitative and quantitative analysis in …

DTT is used as a reducing or "deprotecting" agent for thiolated DNA. DTT is frequently used to reduce the disulfide bonds of proteins and, more generally, to prevent intramolecular and …

Sep 28, 2000 · Herein, we investigated the apoptotic pathways in HL-60 cells triggered by dithiothreitol (DTT), used as a model thiol compound, and tested the hypothesis that thiols …

Jan 31, 2018 · We here develop a single-step assay utilizing dithiothreitol (DTT) to fine-tune the surface coverage of DNA-modified AuNPs. DTT is superior to the commonly used surface …

DTT quenches the fluorescence of the passive reference dye Mustang Purple in Quantifiler HP, causing overestimated DNA concentrations. Purification of DNA extracts containing DTT is …

Dithiothreitol (DTT), also known as Cleland's reagent, is a small-molecule redox reagent. Its oxidized form is a disulfide-bonded 6-membered ring. It is used to break down protein disulfide …

0.25 μM yeast or human CMG, Csm3-Tof1/TIM-TIPIN and Mrc1/ CLASPIN were incubated with 5 μM template DNA and 100 μM AMP-PNP for 30 min at 30°C (yeast proteins) or 37°C (human …

Dithiothreitol [DTT] is also known as Cleland's Reagent. It contains two thiol groups and is commonly used to reduce disulfide bond in proteins. It can protect many thiol-containing …