Immune responses involving CD8⁺ T-cells are severely deficient, providing a model to assess the role of CD8⁺ cells and class I MHC in various experimental systems.

Jan 29, 2021 · After knocking-out surface expression of HLA class I molecules by targeting the B2M gene via CRISPR/Cas9, we also co-expressed a single-chain HLA-E molecule, thereby preventing NK cell fratricide of B2M-knockout (KO) cells via "missing self"-induced lysis.

Mar 1, 2022 · Here, we generated a B2M homozygous knockout somatic cell nuclear transfer-induced embryonic stem cell (SCNT-ESC) line using CRISPR/Cas9-mediated gene targeting. B2M KO cell line, which does not express HLA-I molecules on cell surface, has pluripotency and differentiation ability to three germ layers.

Highly efficient gene editing in primary myeloid cells is made possible by optimized non-viral delivery of CRISPR-Cas9. This enables single and multiplexed gene knockout in monocytes, macrophages, and dendritic cells to advance innate immunity research and cell therapy.

Nov 5, 2024 · SELPLG was found to be upregulated in B2M KO A20 cells compared to B2M WT cells. Tumor growth reduction was observed in both A20 B2M wt and KO cell transplanted groups treated with the SELPLG antibody, with a stronger tumor reduction effect in the KO group.

NCG-B2m-KO is a triple-immunodeficient NCG mouse with a B2m gene knockout. B2M (also known as Ly-m11, beta2m, beta2-m, β2-microglobulin) is a component of the MHC complex essential for MHC I expression. NCG-B2m-KO mice express low levels of MHC I on cell membranes and are resistant to graft versus host disease (GvHD).

敲除B2m基因exon2-3,建立B2m基因敲除小鼠模型。 纯合敲除小鼠在细胞表面上几乎没有任何MHC I类蛋白表达。 CD8+细胞毒性T细胞很少，并且在某些情况下CD4+细胞毒性T细胞代偿性增加。 CD8+T细胞相关免疫应答严重缺陷，可用于评估CD8+细胞和I类MHC的作用。 *使用本品系发表的文献需注明: B2m-KO mice (Cat. NO. NM-KO-18021) were purchased from Shanghai Model Organisms Center, Inc.. Fig1 B2m基因敲除纯合子小鼠CD8 + 细胞毒性T细胞很少，CD4 + T细 …

B2M KO cell line, which does not express HLA-I molecules on cell surface, has pluripotency and differentiation ability to three germ layers. This cell line provides a useful cell source for investigating immunogenicity of allogeneic ESCs and their derivatives for tissue regeneration.

NKG B2m KO mice are constructed by knocking out the B2m gene on the background of NKG mice. Compared with NKG mice, NKG B2m KO mice have a significantly longer survival period after PBMC transplantation and can be used for long-term studies of …

Apr 4, 2019 · As an alternative to HLA homozygous iPSCs, researchers have been using genome-editing technologies to knock out the β2-microglobulin (B2M) gene.