Comparison of alkaline phosphatase (ALP) activity at 7 d after calcification induction using MC3T3-E1 cells in normal (N), Collagen I-coated (Col I), and poly-L-lysine-coated (PLL) culture plates.

Aug 3, 2016 · Alkaline phosphatase expression (ALP) was used to quantify osteoblastic differentiation of MC3T3-E1 cell. Surface roughness was evaluated by a contact profilometer (URFPAK-SV; Mitutoyo, Kawasaki, Japan) and characterized by two parameters: mean roughness (Ra) and maximum peak-to-valley height (Rt).

This study aimed to evaluate the initial adhesion morphology and alkaline phosphatase (ALP) activity of murine pre-osteoblastic MC3T3-E1 cells cultured on anatase/rutile mixed-phase TiO 2 thin films with photocatalytical activity with previously confirmed antibacterial properties.

MC3T3-E1cells were cultured with or without treated materials for 7 days and stained with alkaline-dye mixture provided by an ALP staining kit. A representative photograph of ALP staining is...

Mar 18, 2025 · ALP staining and ARS staining were used to evaluate the osteogenesis process. The scrape-loading dye transfer assay was performed to assess the GJ-mediated intercellular coupling. ... e-f Quantitative analysis of ALP staining in MC3T3-E1 cells. Scale bar = 500 μm. n = 6-8. *P < 0.05, **P < 0.01, ***P < 0.001 compared with the control group ...

Download scientific diagram | (A) Alkaline phosphatase (ALP) staining images of MC3T3-E1 cells after incubation for 7 d; (B) quantitative analysis of ALP activity under normal conditions; and...

3 days ago · Alkaline phosphatase (ALP) and alizarin red (AR) staining MC3T3-E1 cells were seeded in a 12-well plate at 3 × 10 4 cells/mL density. After cell attachment, the medium was replaced with osteogenic induction extracts including sample extracts, 10 mM β-glycerophosphate (Sigma, USA), 0.1 mM dexamethasone (Solarbio, China), and 50 µg/mL vitamin ...

3 days ago · Alkaline Phosphatase (ALP) Activity Assay. To quantitatively assess intracellular ALP activity, commercially available kits were utilized. Samples were placed into a 24-well plate and a 100 μL suspension of MC3T3-E1 cells at a density of 1×10 5 cells was seeded onto the sample surface. After co-incubation for 3 and 7 days, the cells were ...

Sep 19, 2018 · Therefore, we employed a fluorescence readout for quantitative analysis of alkaline phosphatase (ALP) activity using an established murine osteoblast-like cell line, MC3T3-E1 cells and primary...

3 days ago · Alkaline Phosphatase (ALP) Staining and Alizarin Red (AR) Staining. Seven days were spent cultivating the MC3T3-E1 cells in an osteogenic induction medium. PBS was used to wash the cells after they had been fixed with 4% paraformaldehyde for twenty minutes. ... This mixture was used to treat MC3T3-E1 cells. ALP and AR staining results revealed ...