3T3-L1 is a sub clonal cell line derived from the original 3T3 Swiss albino cell line of 1962. The 3T3 original cell line was isolated from a mouse embryo and propagated for this specific line of …

Jun 1, 2012 · In this note, we present a detailed procedure for highly effective and reproducible 3T3-L1 cell differentiation. Due to their potential to differentiate from fibroblasts to adipocytes, …

Differentiation of multi-potential 3T3-L1 fibroblasts into lipid laden adipocyte-like cells is one of the most common in vitro models used in the study of adipocyte biology.

Jan 16, 2025 · The 3T3-L1 cell line, a mouse embryonic fibroblast cell line, was first isolated by Green and Kehinde in 1974. Due to its ability to differentiate into adipocyte-like cells in vitro, it …

The 3T3-L1 cell line is commonly used to study adipocyte biology, adipocyte cell differentiation, and related cellular and molecular mechanisms. The differentiation of 3T3-L1 cells into …

Robust and well-characterized, 3T3-L1 pre-adipocytes differentiate and form fat pads indistinguishable from normal adipose tissue when injected into mice. In this protocol, …

We find that newly thawed, contact inhibited 3T3-L1 cells are adipogenic, but they lose adipogenic potential after being maintained in culture at confluence and losing contact inhibition. Thus, the …

Originally derived from Swiss mouse embryo tissue by Dr. Howard Green of Harvard Medical School, the 3T3-L1 system has been pivotal in advancing the understanding of basic cellular …

3T3-L1 is a mouse pre-adipocyte cell line which can be induced to differentiate into mature adipocytes when it is treated with a chemical cocktail. The most commonly used variant of …

3T3‐L1 fibroblasts are a continuous substrain of 3T3 (Swiss albino) developed through clonal isolation (CL‐173, ATCC). The cells undergo a preadipose to adipose‐like conversion as they …

The 3T3-L1 cell-line is a widely used model system for the analysis of adipocyte differentiation. The characteristic of this cell line is its ability to differentiate into mature adipocytes upon …

Dec 11, 2018 · The 3T3-L1 murine preadipocyte cell line is a commonly used tool for analysis of the subcellular pathways involved in preadipocytic cell differentiation (a process also …

In vitro differentiated 3T3-L1 adipocytes have characteristics similar to tissue-derived adipocytes and have been commonly used to study adipogenesis, lipolysis, and metabolic dysfunctions. …

Matrigel® significantly enhances lipid accumulation in 3T3-L1 cells differentiated on Matrigel®, visualized by Oil Red O staining. 3T3-L1 cells were cultured and differentiated on Matrigel® …

3T3-L1 CAR SNRK KO cells may be used to study the function of SNRK in white adipocytes. The cell line is SNRK deficient and can be induced to differentiate into adipocytes.

Jun 1, 2012 · In this note, we present a detailed procedure for highly effective and reproducible 3T3-L1 cell differentiation. Due to their potential to differentiate from fibroblasts to adipocytes, …

This protocol outlines how to chemically induce the differentiation of 3T3-L1 cells into adipocyte-like cells. 3T3-L1 differentiation is an economical and convenient way to generate adipocyte …

Feb 20, 2025 · In 3T3-L1 adipocytes, RFE inhibited adipogenic differentiation and lipogenesis by downregulating PPARγ (peroxisome proliferator-activated receptor γ), C/EBPα (CCAAT …

3T3-L1 and 3T3-F422A cells are mouse cell lines that are selected clones of fibroblast stocks derived from Swiss 3T3 mouse embryos based on their high adipogenic potential. 3T3-F422A …

Oct 1, 2005 · Expression of Nuclear Receptors during 3T3-L1 Cell Differentiation into Adipocytes. Mouse 3T3-L1 cells were induced to differentiate using two different protocols: DMI (a cocktail …