The use of the Cre/loxP system for generating tissue specific or inducible knockouts is a powerful tool for mouse genetics, but it is only one piece of the total picture. Below, I would to focus on two additional issues: using Cre/lox mice to turn on or off transgenes, and using Cre reporter strains. A Cre switch for transgenes

May 18, 2006 · The FLP-FRT system. The FLP-FRT system is similar to the Cre-lox system and is becoming more frequently used in mouse-based research. It involves using flippase (FLP) recombinase, derived from the yeast Saccharomyces cerevisiae (Sadowski 1995). FLP recognizes a pair of FLP recombinase target (FRT) sequences that flank a genomic region of …

The Cre-lox system is one of the most revolutionary tools in the geneticist's toolbox. You don't have to be a user or developer of cre or floxed mice to appreciate the impact that cre-lox technology has had on biomedical research and genetic engineering.

Conditional mutagenesis using the Cre-lox system is not the only strategy available to spatiotemporally regulate gene expression in mouse models of human diseases. New recombination systems added to the mouse geneticist’s tool box permit further refinement of the Cre-lox system, allowing more sophisticated mouse modeling.

The Cre/lox system can also be used to produce strains in which a transgene is either inducible or expressed only in certain tissues. For example, mating the transgenic strain in Figure 1 to a strain that expresses Cre recombinase in mammary tissue produces double transgenic offspring that express the Kras oncogene only in the mammary glands ...

The Cre/lox system has become the cornerstone of modern mouse genetics because it enables sophisticated control over the timing and location of gene expression. This is the first in a series of posts that looks at some finer points that often go overlooked in experiments with the Cre/lox system. 1. Cre itself can produce a phenotype.

Sep 22, 2011 · More novel and sophisticated uses for the Cre/lox system are being developed all the time. Research mouse models that incorporate the Cre/lox system typically involve generating double, triple, quadruple, or even n-tuple (is that really a word?) mutant/transgenic mice. As you can imagine, breeding such mice can get a bit complicated.

The simplest Cre-lox system to generate a knockout (KO) mouse model has two components that need to be genetically engineered in the murine genome: a target sequence surrounded by loxP sites (often called a “floxed” allele) and a Cre recombinase gene controlled by a specific promoter.

A. Research Tools: Cre-Lox and More FAQ. How do Cre-Lox and Tet-on / Tet-off systems work? Click here for an introduction to Tet Expression Systems. Click here for an on demand webinar on Cre-Lox basics. Additional Cre-Lox resources listed below in the Cre-Lox Resources Section. 2. Does my mouse need to be homozygous for cre?

Dec 8, 2015 · Recombinase-based methods for labeling cells have empowered numerous biological studies of tissue organization, gene expression, and lineage analysis. These strategies commonly employ the cre/lox system in which Cre-mediated recombination removes a loxP-flanked stop codon (lox-stop-lox; LSL) that then allows expression of a downstream reporter, …