signaling the system to initiate gene silencing. "The 'R' in R-loop stands for RNA. All DNA-binding CRISPR-Cas systems use this structure to probe the DNA sequence and identify the correct target ...

A UCO team has applied the genome editing technique, which garnered a Nobel Prize in Chemistry in 2020, to unravel the ...

Gene therapies based on the CRISPR-Cas system have already been successfully used in clinical practice. In recent years, for example, a hundred patients suffering from the hereditary disease ...

“Almost half of all bacteria and the large majority of archaea on planet Earth have an endogenous CRISPR-Cas system in them,” he said. “One of the great things to do is just to repurpose the ...

expanding CRISPRi targeting scope for functional genomics and enabling efficient screening of repeated genomic elements Type I-E CRISPR–Cas system is one of the most extensively studied RNA ...

CRISPR-Cas9 genome editing exploits the CRISPR-Cas system to modify a genome in a targeted manner. Guided by RNA, the Cas9 endonuclease breaks DNA at a target sequence. Imprecise repair of the ...

Yet others, called base editors, change one letter of the DNA code to another. So why do we call it CRISPR? Cas proteins are used by bacteria to destroy viral DNA. They add bits of viral DNA to ...

Australian researchers have successfully introduced an improved version of Cas12a gene-editing enzyme in mice.

Cas9, the first Cas protein repurposed for cutting DNA, introduces double-strand breaks that recruit DNA repair machinery. Despite its successes, the CRISPR-Cas9 system has several limitations, ...

The epigenetic state of chromatin, gene activity, and chromosomal positions are interrelated. A research team from the IPK Leibniz Institute (IPK) and the Karlsruhe Institute of Technology (KIT) ...

Potent in vivo gene editing in skeletal muscle of non-human primates by a novel, ultracompact CRISPR system delivered via a single AAV vector.

Complementary discoveries have the potential to enhance treatment options for antibiotic-resistant infections. Researchers at ...