eLife3d
Kinetic regulation of kinesin’s two motor domains coordinates its stepping along microtubules
This study provides compelling evidence that kinesin's stepping mechanism is governed by strain-induced conformational changes in its nucleotide-binding pockets. Using pre-steady state kinetics and ...
eLife3d
Optogenetic control of Protein Kinase C-epsilon activity reveals its intrinsic signaling properties with spatiotemporal resolution
The activation loops of phosphorylated and T566A mutant PKCε were shown in purple and orange cartoon ... (a) Representative images of HEK293T cells transfected with Opto-PKCɛ and CIBN-GFP-CAAX from ...
Nature1d
Directed evolution by in vitro compartmentalization
Figure 1: Schematic representation of directed evolution by IVC.