To construct the plasmids used in the cell screen, the functional domain was changed to varying architectures of TadA8e, DddAtox(E1347A) or BspD61(C) using AvrII and XbaI for pTracer or BamHI and KpnI ...
ACKR4 Recruits GRK3 Prior to β-Arrestins but Can Scavenge Chemokines in the Absence of β-Arrestins
General plasmids and primers. Nluc-GRK2 was generated by amplifying human GRK2 and Nluc separately, ligating the PCR products over a common ClaI restriction site and cloning it conjointly into the ...
Polyphosphate (polyP) exists in all life forms; however, its biological functions in metazoans are understudied. Here, we explored Drosophila as the first genetic model to explore polyP biology in ...
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