Frizzled (FZD) proteins are the principal receptors of the Wnt signaling pathway. However, whether Wnt ligands induce FZD endocytosis and degradation remains elusive. The transmembrane E3 ubiquitin ...

They were then fixed in 70% ethanol, followed by another PBS wash. Afterward, the cells were stained with propidium iodide (PI) for 30 min in darkness. Flow cytometry was used to analyze the stained ...