The amplicons of the expected size were confirmed with agarose gel electrophoresis. The expected size of the amplicons was 2.7–3.4 kb, and the PCR products of the expected size were confirmed with ...

Paired-End Library Preparation on the MiSeq Platform Amplicon libraries ... All the indexed PCR products were pooled in an equal volume and the pooled libraries were purified by agarose gel ...

COLD-PCR amplification yields amplicons containing enriched proportions of mutant (or variant) alleles, thus permitting the discrete detection and identification of minority alleles by downstream ...

The E-Gel ® electrophoresis system is ideal for routine analysis of PCR products, restriction digests, and plasmid preparations. No gels to pour, no buffer to make, no staining/destaining ...

COLD-PCR exploits a small but critical difference in amplicon melting temperature. COLD: Coamplification at lower denaturation temperature; RS: Reference sequence; T c: Critical denuration ...

School of Biology & Environmental & Biological Sciences, University College Dublin, Belfield, Dublin D04 N2E5, Ireland UCD-Centre for Food Safety, University College Dublin, Belfield, Dublin D04 N2E5, ...

Department of Clinical Laboratory of Sir Run Run Shaw Hospital, College of Biosystems Engineering and Food Science, Zhejiang University School of Medicine, Hangzhou 310058, People’s Republic of China ...

1Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NE 68198-5900 USA 2Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical ...

This important study reports a detailed quantification of the population dynamics of Salmonella enterica serovar Typhimurium in mice. Bacterial burden and founding population sizes across various ...