To construct the plasmids used in the cell screen, the functional domain was changed to varying architectures of TadA8e, DddAtox(E1347A) or BspD61(C) using AvrII and XbaI for pTracer or BamHI and KpnI ...

all the UNC-13 expression constructs were amplified by PCR and inserted into JB6 vector between the KpnI and NotI sites. Red fluorescent protein mApple was inserted between NotI and MluI sites ...

The resulting clone was designated pCMV3-SΔ19. For the generation of a lentiviral vector containing SARS-CoV2–SpikeΔ19, the pCMV3-SΔ19 insert was initially digested with KpnI and blunt polished using ...

A growing number of evidences has emerged over the last decade implicating that cells organize a plethora of biochemical processes by means of biomolecular condensation, including the formation of ...