This important study focused on characterizing clonally derived MSC populations from the synovium of normal and osteoarthritis (OA) patients, demonstrating their potential to regenerate cartilage in ...

The medium was changed every third day. Cells were continuously passaged every 5–7 days at 90–100% confluency until stable culture phenotype (usually 7–9 weeks). Cell suspension was diluted 4–6-fold ...

Cell culture medium for human microvascular endothelial cells ... SF and human primary chondrocytes were grown up to 70–80% confluency before stimulation. Human endothelial cells were stimulated at a ...

(ii) If possible, conduct iPSC reprogramming and maintenance in low O 2 (5%) culture conditions. (iii) Never over-grow iPSC cultures: passage the cells when the cells reach 60–70% confluency or on day ...

LX-2 cells were cultured in 12-well plates until confluency to determine their wound-closure capacity ... 30 In vitro activation of HSCs was achieved by cell culture on uncoated tissue culture dishes.

Cell-to-cell communication is essential throughout all forms and stages of life, and many communication mechanisms are well ...

In December 2023, through the development efforts of CRISPR Therapeutics and Vertex Pharmaceuticals, their decades-long ...

Cancer cells work together to source nutrients from their environment -- a cooperative process that was previously overlooked by scientists but may be a promising target for treating cancer.