According to publications, high siRNA concentrations during transfection may result in non-specific off-target effects. Using as little siRNA as possible can improve the reliability of the ...

We demonstrate the optimization of siRNA transfection process for time and costs in a high throughput application with two independent measurements of the RNAi effect: The visual monitoring of Eg5 ...

siRNA transfection is a powerful tool to understand underlying mechanisms in gene regulation and molecular pathways. Transfecting siRNA with a high efficiency while minimizing off-targets and side ...

Polyplus-transfection has announced the launch of jetPRIME ™, a new reagent for DNA and siRNA transfection. jetPRIME™ brings pharmaceutical and biotechnology researchers a number of advantages for day ...

Therefore, the purpose of this study was to compare siRNA transfection efficiency at different time points in various target cells of the inner ear, including hair cells of the Organ of Corti ...

It works with all RNA types (mRNA, siRNA, and miRNA) according to Dr. Mahon. “We are seeing levels of transfection of 95 to 100 percent with no toxicity. We are also able to do repeat ...

Details of each trial can be found on www.clinicaltrials.gov. † Ongoing trials. AMD: Age-related macular degeneration; DME: Diabetic macular edema; iv.: Intravenous ...

The practical application of siRNA will be covered through discussion of best-practice for experimental design. This will include proper use of positive and negative controls, optimization of ...

There are also continuing challenges with the intracellular delivery of siRNA. Common methods for delivery include transfection, electroporation, and viral-mediated delivery. The most widely ...

Transfection can be stable or transient. Stable transfection results in sustained, long-term expression, whereas expression will eventually be lost after transient transfection as host cells replicate ...