FRAP experiments performed at 37 °C with soluble GFP targeted to the ER result in a 1.4-fold faster recovery rate than at 23 °C, owing to differences in viscosity 28. The diffusion coefficient ...

and unassembled GFP-fragment fusions are typically insoluble, the reassembled fusion can be purified and concentrated in a single step from the soluble fraction of cell lysate 7. Combine the ...