Restriction enzyme digestion continues to be one of the most common techniques used by researchers who carry out DNA cloning experiments.

The mutation is discriminated by digestion with specific restriction endonucleases and is identified by gel electrophoresis after staining with ethidium bromide (EtBr). This convenient and simple ...

During this week you will carry out restriction digestion of the genomic clone, in order to cut the insert (=the gene) from the plasmid DNA (=vector). Following separation of insert and vector by ...

In short, LUMA uses DNA restriction digest reactions followed by pyrosequencing to analyze methylation. Methylation percentage is based on methylation sensitivity to different restriction enzymes.