In higher eukaryotes, the dynamics of replisome components during fork collapse and restart are poorly understood. Here we have reconstituted replication fork collapse and restart by inducing ...

Purification of replisome protein complexes through protein-protein interactions is useful to identify potential components, but it provides limited spatial information about protein localization.

Cdc45 and GINS recruit and tether other replisome components to the helicase, including the DNA polymerases that copy each strand of the DNA.

coli fixes damage to DNA in the middle of duplicating it. The machinery that duplicates E. coli DNA is called the replisome, said Tuan Pham, project scientist in the UC Davis Department of ...