This method allows cloning with low amounts of DNA and for high throughput applications. PCR for next-generation sequencing (NGS) PCR amplification allows the creation of multiple NGS libraries. This ...

or in the case of multiplexed RT-PCR, lack viral sequence information. Next-generation sequencing (NGS) hybrid capture combines high-throughput capabilities with high sensitivity, helping scientists ...

NGS is still predominantly used in a research ... [37] To achieve this, the selected target regions must be PCR amplified, quantified and pooled in equimolar amounts, hereby generating target ...

NGS uses a single tube reaction, taking advantage of the conserved sequences on the 5´ and 3´ ends of each viral RNA. PCR products are rather large, but by employing the Nextera Tagmentation ...

Both Sanger sequencing and next-generation sequencing (NGS) are powerful tools for genomic analysis that are commonplace in labs around the world. Choosing the best technique is important and will ...

One of UMass Lowell’s Core Research Facilities, the Next Generation Sequencing & Genomics Lab provides Next-Generation ... DNA & RNA isolation, PCR/qPCR, Qubit fluorometry, gene expression and drug ...