GSTP1 genotype was determined by Pyrosequencing ... The Netherlands), Sepharose beads from Amersham (Uppsala, Sweden). PCR reactions were performed using Hotstart PCR mastermix (Qiagen, Hilden ...

From each sample, the researchers took DNA, amplified it using polymerase chain reaction techniques, and looked for reactions indicating the presence of methylation. Through direct DNA sequencing, ...

PL converts to hPL, an active drug that silences a gene called GSTP1. The GSTP1 gene produces a detoxification enzyme that is often overly abundant in tumors. "We are hopeful that our structure ...