Lukyanov, of the Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, and colleagues report the isolation of a GFP variant called KillerRed that acts as a ...

Their improved-folding variant contained six new mutations, and they termed it superfolder GFP. As expected, the color variants of GFP could also be made from superfolder GFP, but the method is ...

and it produced GFP variants with different colours and capacities for changing colour in response to environmental stimuli. With the cloning of new fluorescent proteins from species other than ...

The GFP reporting system was then used to test the specificity of these variants. The variant ChCas12b-D496A showed less off-target activity according to GUIDE-seq analysis, which might make it a ...