You may have seen it in the news recently: a baby in Pennsylvania with a rare genetic disorder was healed with a personalized ...

Here we present a method that we have named ligation-mediated purification, which is based on purification of enzymatically cleaved DNA, followed by its quantification (see Fig. 1 for a general ...

Accordingly, the total loss of DNA ligase function is lethal. DNA ligases catalyze the joining of a 5’-phosphate-terminated strand to a 3’-hydroxyl-terminated strand. Ligation depends on magnesium and ...

Although the approaches they take are quite different, all of them rely on some combination of two key biochemical reactions: DNA ligation ... different ways a DNA strand can be broken.

A unique feature of HR is that it repairs the damage to the DNA in a highly accurate way. In eukaryotic cells (those with a ...

Prime editing is an advanced gene editing technology that enables precise DNA corrections without introducing double-stranded breaks, significantly reducing off-target effects. This innovation holds ...

Scientists have uncovered a critical role for rapid DNA repair in maintaining genome stability. A new study reveals that ...

To barcode samples, the research team established a tailing and single-strand ligation method that utilizes terminal deoxynucleotidyl transferase (TdT) to tail the 3’ terminus of DNA in DNA ...