Although the approaches they take are quite different, all of them rely on some combination of two key biochemical reactions: DNA ligation ... different ways a DNA strand can be broken.

Accordingly, the total loss of DNA ligase function is lethal. DNA ligases catalyze the joining of a 5’-phosphate-terminated strand to a 3’-hydroxyl-terminated strand. Ligation depends on magnesium and ...

Here we present a method that we have named ligation-mediated purification, which is based on purification of enzymatically cleaved DNA, followed by its quantification (see Fig. 1 for a general ...

To barcode samples, the research team established a tailing and single-strand ligation method that utilizes terminal deoxynucleotidyl transferase (TdT) to tail the 3’ terminus of DNA in DNA ...