Numerous DNA binding viability dyes can be used in the annexin V assay including PI, DAPI and DRAQ7 (Biostatus). The Millipore Muse mini flow cytometer can be used to determine apoptosis levels in a ...

Cell apoptosis was detected by flow cytometry with Alexa Fluor 488 annexin V and propidium iodide fluorescence staining (Molecular Probes, Eugene, Oregon).

The autophagic inducing agents, chloroquine and curcumin also induce apoptosis and thus PS is externalized to the outer leaflet of the plasma membrane and thus the annexin V assay can be used to ...