Yet even if the outputs of these various algorithms were the same, the profile-based algorithms that incorporate the secondary structure of the 16S rRNA gene are significantly faster than those ...

When analyzing 16S rRNA using PCR, a universal primer is applied to the sample. The partial 16S gene amplicons that results from this contains the hypervariable regions, which is compared to other ...

To achieve this, the researchers designed a small ribozyme-based RNA molecule (also called catalytic RNA) that attached a unique barcode to 16S rRNA upon gene transfer. This cat-RNA was introduced ...

Since the beginning of the microbiome era decades ago, 16S rRNA gene-based amplicon sequencing has been widely adopted for microbial composition profiling. Many researchers might assume the 16S ...

DNA was extracted and either the V4 region of the 16S rRNA gene was amplified and sequenced on an Illumina MiSeq or the V1 to V9 regions were amplified and sequenced on a PacBio Sequel II. During the ...

Compared with shotgun sequencing, this is more significant for 16S rRNA amplicon sequencing. For 16S rRNA gene sequencing, it is supposed to set a quality threshold as high as possible and to trim ...

Sequence analysis of 16S ribosomal RNA identified several hundred bacterial species of the intestinal ecosystem, most of which cannot be cultured. Objectives We aimed to evaluate gut microbiota ...

To achieve this, the researchers designed a small ribozyme-based RNA molecule (also called catalytic RNA) that attached a unique barcode to 16S rRNA upon gene transfer. This cat-RNA was introduced ...